Inhibition of Angiotensin Converting Enzyme (ace) by Viola Mandshurica Extraction

Angiotensin-converting enzyme (ACE, EC 3.4.15.1) inhibitor plays a critical role in treating hypertension by causing blood vessels to constrict. The purpose of this study was to estimate ACE inhibition activity by Viola mandshurica using an in vitro assay. The assay method is based on the hydrolysis of the substrate HHL by ACE, and measuring the amount of HA using RP-HPLC. ACE inhibitor activity was evaluating by determining the degree of hydrolysis rate of substrate, hippurl-L-histidyl-L-leucine (HHL). At 240 nm, the absorbance of treatment groups was the highest in V. mandshurica and HHL. More exactly, the absorbance ratio of HHL and extracts from V. mandshurica at 228 nm was high than that at 240 nm. The standard control reagent, captopril was used as a positive control for ACE inhibition. The extraction of V. mandshurica leaves showed inhibition activity more than 39.1%. The extraction of V. mandshurica petioles showed inhibition activity more than 28.7%. The roots of V. mandshurica demonstrated ACE inhibitory activity at a concentration of 20%, showing an inhibition greater than 46%. The roots extraction for ACE inhibitor was more effective than leaf and petiole extractions. The results of this study suggest that the root extraction of V. mandshurica can be utilized in further studies as one of high ACE inhibitory effectors.